Monday, October 5, 2009

First WEek of InternShip

My first week of being an intern at AUFMC, is just like our preceptorship day. We were not yet assigned in our permanent sections in the lab because Ma'am Noemi is on --- leave (sorry, im not allowed to say the word :)) hehe) So we waited until she came and give us our partner and our section. My partner in Phlebotomy section is non other than--Shane! Cant believe that she would be my lab partner for 6months.

At first we were both nervous in extracting blood from patients, but of course we dont have a choice, we cannot endorse every extraction to our staff and the fact that we are there to learn. And so now, on my third week in that section, I'm well adjusted with the works there. We also join our staff in warding, we usually have 5AM, 6AM, 10AM, 8PM, and 12MN hours of warding.

Night duties are the "magastos" duty that we have. Because we usually eat at least 4x from 1PM-8AM of duty. We are lucky if we have 4hrs of sleep, from my last duty I only have an hour for nap time. And always expect that you have loads of specimen in the morning specially when its weekdays. It's not toxic in the lab during weekends.

The staff there are good, we have junior and senior staff. Most of the staff there are former students in AUF, so we share common stories during dull time.

Tuesday, September 1, 2009

Drug Testing

" Do you agree that drug testing should be done as a requirement for admission to schools? Why and why not?"

I agree that drug testing should be included as one of the tests to be accomplished by an incoming student when enrolling in a university or school.

Let's face it, not all freshmen are first timers in college, some are transferees from other schools. It's a another way of checking the background or personality of individuals.

Taking drugs like marijuana and cocaine which are substances of abuse is not something to be proud of. People using these (for me) are people who have problems and are not capable of handling them.

No one wants to be in a school with classmates who are drug users in the first place. And for parents, they don't want their children to be exposed in that kind of environment. They brought their children to school to learn good things and not to be influenced to the wrong direction.

And if you're the school owner, you don't want your school to be call "School of Addicts", it would be a greatly disgrace if you have students who are drug users just because they want to be happy. So I really agree of drug testing as a part of admission test for an incoming student.



1. EIA (Enzyme Immunoassay)
2. True
3-5. Unknown (antigen), Antibody (enzyme linked), substrate
6-8. Sandwich ELISA, Competitive ELISA, Indirect ELISA
9. True
10. True

CASE Analysis

The doctor might order a test to determine the calcium level in the womans body including the hormones that regulate the calcium.

ELISA is recommended method in determination of hormone levels. Because its a direct measurement, and a very specific method.

Tuesday, August 25, 2009

CasE AnaLysiS for ELISA

1. Other name for ELISA.

2. True or false: The principle of ELISA is to detect an unknown sample using a specific antibody which is linked in an enzyme, which the antigen will react with, that will be measured directly.

3-5. What are the reagents used in ELISA?

6-8. Give at least three example of ELISA.

9. True or false: Elisa can also be used in measuring hormone?

10. True or false: Can ELISA be considered a confirmatory test?

CaSE AnaLysis

A 60 y.o. woman is experiencing backache, broken bones or fracture, decrease in height, and was diagnosed to have a mild osteoporosis 10years ago. Her doctor gave her already medicines and she started to recover from it. Unfortunately, due to insufficient money, she failed to complete her medications and begun to work as a laundry woman in a rich family. Because the family loved her, they sent her to a doctor and promised to support her entire medication.

What is the possible test her doctor might request for her?

Give one method which can confirm test results.



Sunday, August 9, 2009

FeeLinG LucKY!!

WooooH! I cannot believe it, I won a hundred dollar from the raffle in the Top 10 Emerging Influential Blogs 2009 Contest.

Congrats for all the winners, the Top 10 Influential Bloggers and the other lucky persons who were picked in the lottery bowl.

Thank you so much for the sponsors and ofcourse Maam Janette.=)

Thursday, August 6, 2009



Enzyme-linked Immunosorbent Assay, as the name implies, it uses enzyme, antigen, and antibody reaction. It is also known as EIA, or Enzyme Immunoassay. It can measure antigen-antibody binding. You can use it in measuring hormones, enzymes etc.

Principle: Unknown antigen is attached in a cell surface, then you will add a specific antibody to which that antigen will react to (you now have antigen-antibody reaction)this antibody is linked to an enzyme. To have a measurement of the antigen-antibody reaction you need to add a substance which can cause the enzyme to produce a detectable signal, like an amplifier. (e.g. Fluorescense ELISA)

There are several types of of ELISA:

Indirect ELISA and Sandwich ELISA

Competitive ASSAY

In relation to parathyroid hormone, ELISA can be used to measure the amount of PTH in our system. As mentioned in the intorduction, it can measure hormones. You can apply any of the severeal types of ELISA procedure. ELISA kit are now available in the market for easy use.


Images: biology.arizona


Wednesday, July 29, 2009

Top 10 Blogs

ToP 10 iNfLuEntiAL BlogS of 2009

They say writing is a passion. But for these bloggers, writing is not just their passion, and not just a hobby, but their own way of expressing themselves, their thoughts, and ideas. And now presenting The 10 Most Influential and Inspiring blogs:

1. Zorlone

A doctor by profession, specialist in Internal Medicine. Continues to share his talent and inspire people - for those who read his blog.

2. The Struggling Blogger

Despite life's challenges, he still remains strong. He helps other people without asking for something in return. You would see inspiring poems and articles in Roy dela Cruz's blog.

And he wrote a post for us which had helped us a lot to be known in the blogging world. Dakal pung salamat Sir Roy,

3. Jan Geronimo of WritingToExhale

A writer who pens from the heart. The emotions dripping from his pen inspires and motivates me to become a better person.

4. Angel Cuala of Father Blogger dot com

A responsible family man. Fathers should emulate him for his love for his family. A great blogger!

5. Irene of LifeLots

Good articles are found in her blog. As a student, her thoughts are sources of inspiration for me.

6. Luke of A Walk In The Dark

The title of the blog is far from his posts. I feel a warmth from the person who is behind the powerful articles. His first post got so many comments.

7. Holly Jahangiri of It’s all a matter of Perspective: Mine

Down-to-earth woman. Her posts are candid and straightforward.

8. Bingkee of I Love/Hate America

She writes with no fear. I admire her audacity and zest for life.

9. Kelvin Servigon of Kelvinonian Ideas 2.0

He could be a model for us. In his young age he made a name for himself in the blogging world.

10. Dee of Tales From The Mom Side

She has witty and informative posts deserved to be read.

To all my nominees, Goodluck. May you all continue to inspire people. God is with you.

Before I forget , these are the sponsors: (I want to win

Absolute Traders, My Brute Cheats, Business Summaries, Fitness Advantage Club, Events and Corporate Video,, Dominguez Marketing Communications, Red Mobile, Budget hotel in Makati, and Lucio C. Tan Group of Companies.