CasE AnaLysiS for ELISA

1. Other name for ELISA.

2. True or false: The principle of ELISA is to detect an unknown sample using a specific antibody which is linked in an enzyme, which the antigen will react with, that will be measured directly.

3-5. What are the reagents used in ELISA?

6-8. Give at least three example of ELISA.

9. True or false: Elisa can also be used in measuring hormone?

10. True or false: Can ELISA be considered a confirmatory test?

CaSE AnaLysis

A 60 y.o. woman is experiencing backache, broken bones or fracture, decrease in height, and was diagnosed to have a mild osteoporosis 10years ago. Her doctor gave her already medicines and she started to recover from it. Unfortunately, due to insufficient money, she failed to complete her medications and begun to work as a laundry woman in a rich family. Because the family loved her, they sent her to a doctor and promised to support her entire medication.

What is the possible test her doctor might request for her?

Give one method which can confirm test results.

Reference:

Picture:

flckr.com

Others:
Health.yahoo.com

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ELISA

ELISA

Enzyme-linked Immunosorbent Assay, as the name implies, it uses enzyme, antigen, and antibody reaction. It is also known as EIA, or Enzyme Immunoassay. It can measure antigen-antibody binding. You can use it in measuring hormones, enzymes etc.


Principle: Unknown antigen is attached in a cell surface, then you will add a specific antibody to which that antigen will react to (you now have antigen-antibody reaction)this antibody is linked to an enzyme. To have a measurement of the antigen-antibody reaction you need to add a substance which can cause the enzyme to produce a detectable signal, like an amplifier. (e.g. Fluorescense ELISA)

There are several types of of ELISA:

Indirect ELISA and Sandwich ELISA















Competitive ASSAY

















In relation to parathyroid hormone, ELISA can be used to measure the amount of PTH in our system. As mentioned in the intorduction, it can measure hormones. You can apply any of the severeal types of ELISA procedure. ELISA kit are now available in the market for easy use.


Reference:

Images: biology.arizona
ncbi.nlm
mdsystem.com

Others: wikipedia.com